Fig 1a. LB Agar Amp plate of BL21(DE3) E. coli bacteria with DNA plasmid pGEM-gbr22 and SOC media after 14 hours in a 37°C incubator.
Fig 1b. Control LB Agar Amp plate of BL21(DE3) E. coli bacteria with SOC media, without DNA plasmid after 14 hours in a 37°C incubator.
Fig 1c. LB Agar Amp plate from another group's BL21(DE3) E. coli bacteria with DNA plasmid pgbr22 and SOC media after about 14 hours in a 37°C incubator. Colonies used for starter culture were derived from this plate.
Fig 1d. LB Agar plate (no Amp) of a swab from Tyler’s belly button after 14 hours in a 37°C incubator.
Fig 2. Two flasks of LB and 100ug/ml ampicillin with BL21(DE3) E. coli bacteria transformed with DNA plasmid pgbr22 after 24 hours in a 37°C shaking incubator. Purple color originated from purple protein produced by bacteria.
Fig 3. Purple cell pellets of BL21(DE3) E. coli bacteria transformed with DNA plasmid pgbr22 after 10 minutes in a 4°C centrifuge (5,000 rpm); excess liquid decanted.
Figure 7. Elutions 1 and 2 of pgbr22 released from Ni-NTA chromatography resin (using 1xPBS and 250mM imidazole elution buffer).
Figure 8. SDS-Page gel of 6 samples of pgbr22 prior to drying. Columns right to left: ladder, cell lysate, soluble fraction with pellet, flow through, wash (using 20mM imidazole), elution 1, and elution 2 (both elutions done with 1xPBS and 250mM imidazole). Pre-stained protein ladder #26616 used as standard.
Figure 9. Dried SDS-Page gel of 6 samples of pgbr22. Columns 1-6: cell lysate, soluble fraction with pellet, flow through, wash (using 20mM imidazole), elution 1, and elution 2 (both elutions done with 1xPBS and 250mM imidazole). Pre-stained protein ladder #26616 used as standard.
Introduction:
Materials & Methods:
Results:
Conclusions:
References: